Assembly of Nano?Biocatalyst for the Tandem Hydrolysis and Reduction of p?Nitrophenol Esters

Hybrid nano-biomaterials are exploited in the design and performance of chemo-enzymatic cascades. In this study, lipase is immobilized from Candida antarctica fraction B (CALB) gold nanoparticles (Au NPs) on magnetic particles coated with silica ([email protected]2) to stepwise hydrolyze reduce p-nitrophenyl esters tandem reaction. The assembly two catalysts at interface [email protected]2 temporal control reaction turns out be most determinant parameters for cascade kinetics. When both CALB Au NPs co-immobilized particle, reactions take place significantly faster than when physically segregated by their immobilization different particles. Herein, it demonstrated that co-immobilization biocatalysts nanocatalysts solid materials creates hybrid interfaces accelerated reactions.